Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2018
  • Volume: 52
  • Issue: 3

First report on optimization of loop-mediated isothermal amplification (LAMP) for the diagnosis of Babesia felis

  • Author:
  • Muhammad Awais Salim1, Raheela Akhtar1,, Muhammad Lateef1, Muhammad Imran Rashid1, Haroon Akbar1, Wasim Shehzad2, Fareeha Akhtar3, Saher Islam1, Shahid Hussain Farooqi1, Uzma Farid Durrani4
  • Total Page Count: 4
  • Page Number: 401 to 404

1Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan

2Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan

3University Diagnostic Lab, University of Veterinary and Animal Sciences, Lahore, Pakistan

4Pet Centre, University of Veterinary and Animal Sciences, Lahore, Pakistan

Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan

*Corresponding author's e-mail: raheela.akhtar@uvas.edu.pk

Online published on 4 April, 2018.

Abstract

The objective of present study was to optimize loop mediated isothermal amplification (LAMP) assay for the diagnosis of Babesia felis in cats. LAMP primers were designed recognizing four sections of 18SribosomalRNA (18S rRNA) gene of B. felis. The blood samples of cats microscopically positive for Babesia felis were further used to extract deoxyribo neuclic acid (DNA) and the reaction mixture of 25 μL was standardized at 63°C temperature for 1 hour. LAMP assay provided more positive samples than conventional polymerase chain reaction (PCR). The prevalence of B. felis was also determined in cats using this optimized LAMP assay and it was found that the prevalence was more in younger cats as compare to adults. The application of LAMP can be helpful in rapid, reliable and cost effective diagnosis of B. felis in field.

Keywords

Babesia felis, Diagnosis, LAMP assay, Optimization, Prevalence