Rapid detection of infection due to Mycoplasma mycoides subsp. capri in experimental goats by PCR by assay

Mycoplasma causes a number of important disease viz., CBPP, CCPP, CRD, Arthritis, Contagious agalactiae and Mastitis in livestock and poultry. The conventional method of isolation and identification of the causative agents of these diseases are generally employed in conjunction with the serology which have the limitation of cross reactivity with other mycoplasma and poor growth of the organisms. To overcome these problems, the PCR assay which is rapid, sensitive and specific was used in the present study to detect the mycoplasma infection due to M. Mycoides subsp.capri directly from the tissue materials viz., lungs, lymphnodes, spleen and liver from the experimentally infected goats. All the tissue materials were subjected to PCR assay with cluster specific primers (MC 323 and MC 358), which yielded approximately 1.5 kbp long product. Further,. It was characterized by using mycoides group specific primers (MM 450 and MM 451) and Mycoplasma capri specific primers (P 4 and P 6) which gave an amplicons of 574 bp and 195 bp products, respectively. The findings indicate that the PCR assay is very simple and useful method for detecting the mycoplasma infection directly from the tissue materials in a very short span.