Siirt University, Faculty of Veterinary Medicine, Division Basic Sciences, Department of Biochemistry, Siirt, Turkey
*Corresponding author's e-mail: m.oguzhankaya@gmail.com, oguzhan@siirt.edu.tr
Online published on 17 September, 2018.
In this study, the in vitro effects of enrofloxacin, a broad spectrum antibiotic, and 2, 4-Dichlorophenoxy acetic acid dimethylamine salt that, a herbicide, on the bovine testicular hyaluronidase (BTH) were analyzed. BTH was purified using the method of ammonium sulfate precipitation and using affinity gel (Sepharose-4B-L-tyrosine-m-anisidine). Then the inhibiting effects of enrofloxacin and 2, 4-Dichlorophenoxyacetic acid dimethylamine salt onthe purified BTH enzyme were identified. IC50 values of these chemical substances were found to be 0.3101 and 0.4276 mM, respectively.
Antibiotic, Herbicide, Hyaluronidase, IC50, Inhibition
