Indian Journal of Animal Research

SCOPUSWeb of Science
  • Year: 2019
  • Volume: 53
  • Issue: 11

Immunohestochemical staaning of cd3, cd79αcy and s-100 on bursa fabricius, thymus and spleen of turkeys (Meleagris gallapavo)

  • Author:
  • Tolunay Kozlu1,, Ebru Karadag Sari2, Yesim Akaydin Bozkurt1, Nevin Kurtdede3
  • Total Page Count: 5
  • Page Number: 1450 to 1454

1Department of Histology and Embryology, Faculty of Veterinary Medicine, Mustafa Kemal University, Hatay, Turkey

2Department of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey

3Department of Histology and Embryology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey

Department of Histology and Embryology, Faculty of Veterinary Medicine, Mustafa Kemal University, Hatay, Turkey

*Corresponding author's e-mail: tolunaykozlu@hotmail.com

Online published on 28 January, 2020.

Abstract

Distribution of CD3, CD79ácy and S-100, immunohistochemically, in the bursa of Fabricius, thymus and spleen has not been reported in turkeys. Therefore, we determined the localisation of anti-CD3 protein antibodies for mature T-lymphocytes, anti-CD79αcy antibodies for B-lymphocytes and anti-S-100 protein antibodies for follicular dentritic cells in the bursa of Fabricius, thymus and spleen, which are the lymphoid organs, in a study sample of turkeys. Triple staining method was applied to demonstrate the general structure. Moreover, in all the organs positive reactions were observed with the CD3, CD79αcy and S-100 antibodies. It was also found that similar areas had a positive reaction with CD79αcy and S-100 in all of the tested organs. It was remarkable that CD79αcy reacted positively on Hassall's corpuscles (strongly) and the reticular cells (weakly) in the medulla of the thymus instead of the B-lymphocyte positive areas.

Keywords

CD3, CD79 αcy, Lymphoid tissue, S-100, Turkey