Department of Microbiology, College of Veterinary Science, Khanapara-781 022, Guwahati, India.
The molecular epidemiological study of foot-and-mouth disease virus (FMDV) has been carried out from different outbreaks in Assam the present study is based on the nucleotide sequencing of circulating FMDV serotype. The samples were subjected to sandwich ELISA, multiplex-PCR and molecular phylogeny to identify the type species. The phylogenetic analysis of virus sequence revealed similarity with the Bangladesh isolates in the major branching pattern. The serotype ‘O'has found to be dominant and responsible for most of the recent out breaks. The persistence of serotype ‘O’ and cytokines expression of IL-1α, IL-1β, IFN-α, TNF-α in blood of recovered animals were done by Real time PCR. The findings indicated that IL-1α, IFN-α and TNF-α genes were up-regulated upto 3 months post infection but IL-1β found to be down regulated with progression of recovery. The present study thus supports that real-time PCR is a powerful technique for reliable detection of persistent FMDV in recovered animals.
Assam, Cattle, Foot and mouth disease virus type, Molecular epidemiology, Phylogenetic analysis
