Indian Journal of Animal Research

SCOPUSWeb of Science
  • Year: 2019
  • Volume: 53
  • Issue: 5

Nanoparticle based Brucella melitensis vaccine induced oxidative stress acts in synergism to immune response

  • Author:
  • Amit Kumar, V.K. Gupta1, Anu Rahal2,, Rajesh Mandil3, A.K. Verma4, S.K. Yadav
  • Total Page Count: 7
  • Page Number: 648 to 654

Department of Veterinary Microbiology, College of Veterinary Sciences, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura-281 001, Uttar Pradesh, India

1CADRAD, Indian Veterinary Research Institute, Izatnagar-243 122, Uttar Pradesh, India

2Division of Goat Health, Central Institute for Research on Goats, Mathura-281 122, Uttar Pradesh, India

3Department of Veterinary Pharmacology, College of Veterinary Sciences, DUVASU, Mathura-281 001, Uttar Pradesh, India

4Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Sciences, DUVASU, Mathura-281 001, Uttar Pradesh, India

Abstract

Brucella melitensis, mainly responsible for human brucellosis, is considered as biological warfare. Being intracellular in nature Brucella induces oxidative stress due to bacterial invasion in organs like spleen and lymph nodes. The present study was aimed toevaluate the specific oxidative stress responses in erythrocytesand tissues induced byintranasal inoculation of killed Brucella whole cell protein antigen coupled with sodium acrylate nanoparticle adjuvant. Glutathione mediated nonenzymic antioxidant defense system and catalase have been observed to play a critical role in intracellular antioxidant defenses in vaccinated animals and also in maintaining an excellent milieu to induce an active immune potential. The antioxidant defenses in erythrocytes, liver, kidneys, lungs and spleen of vaccinated mice also maintained excellent redox homeostasis required for quality and effective protection without inflammatory pathophysiology.

Keywords

Brucella melitensis, Immune response, Nano particle, Oxidative stress