Indian Journal of Animal Research

SCOPUSWeb of Science
  • Year: 2019
  • Volume: 53
  • Issue: 6

Expression study of fertility related genes with respect to sperm motility, HOST and acrosome integrity in bovine semen

  • Author:
  • I. Borgohain, D. Dutta, P. Borah, D. Borah
  • Total Page Count: 4
  • Page Number: 716 to 719

Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Khanapara-781 022, Assam, India

Abstract

The present study was conducted for molecular evaluation of bull semen and to study its relationship with different semen characteristics. Fresh semen samples were collected from six breeding bulls. A total of six ejaculates were collected from each of six healthy breeding Jersey bulls at 4 days interval. Immediately after collection, the samples were subjected to physio-morphological and biomolecular evaluation. Percentage of Hypo-osmotic Swelling Test (HOST) positive spermatozoa and acrosome-intact sperms increased with an increase in initial sperm motility. Expression of Chaperonin Containing T-Complex protein 1, subunit 8 (CCT8) gene was found to be negatively correlated with sperm motility, whereas the expression of Adenylate Kinase 1 (AK1) genedid not showany significant relationship with sperm motility.

Keywords

Adenylate Kinase 1(AK1), Breeding bulls, Hypo-osmotic Swelling Test