Indian Journal of Animal Research

SCOPUSWeb of Science
  • Year: 2019
  • Volume: 53
  • Issue: 8

Molecular characterization of universal Mycoplasma and Acholeplasma laidlawii from buffaloes (Bubalus bubalis) at Karachi, Pakistan

  • Author:
  • Syed Khurram Fareed1, Faiz Muhammad1, Javed Muhammad2, Masood Rabbani2, Taseer Ahmed Khan3, Allah Bux Kachiwal4, Shakeel Ahmed Khan1, Aqeel Ahmad1,
  • Total Page Count: 5
  • Page Number: 1080 to 1084

1Department of Microbiology, University of Karachi, Karachi, Pakistan

Department of Microbiology, University of Karachi, Karachi, Pakistan

2University Diagnostic Laboratory, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

3Department of Physiology, University of Karachi, Karachi, Pakistan

4Department of Clinical Medicine and Surgery, Sindh Agriculture University, Tandojam, Pakistan

Abstract

The presentstudy was conducted to identifyand characterize the bovine Acholeplasma laidlawii from 1120 clinically ill buffaloes and 470 lungs collected from buffaloes at abattoir of Karachi. All buffaloes were clinically examined and around 467 (13.48%) buffaloes had signs of respiratory distress including sticky nasal discharge, sunken eyes, dyspnea and fever. All the culture positive samples were further evaluated by PCR using specific primers of A. laidlawii and the amplified product to re-validate the sequence of the specie. This revealed that suspected buffaloes, 92 (2.66%) from nasal discharge and 44 (4.03%) from lung tissue samples respectively were positive for A. laidlawii. Rests of the samples were still under investigation. However it is first time proclaimed on research basis that A. laidlawii was infecting the buffaloes in Pakistan and precautionary measures should be taken to prevent losses from this specie in the months of October and April.

Keywords

Acholeplasma, Buffaloes, Clinical symptoms Mycoplasma, PCR