Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2020
  • Volume: 54
  • Issue: 1

Phenotypic and genotypic characterization of mecA and blaZ gene mediated antibiotic resistance in bovine mastitis due to Staphylococcus spp.

  • Author:
  • Jibin Jose, Surya Sankar, Binu K. Mani, M.P. Unnikrishnan, Anu Bosewell, M. Mini
  • Total Page Count: 3
  • Page Number: 125 to 127

Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy Kerala Veterinary and Animal Sciences University, Pookode, Wayanad-673 576, Kerala, India

*Corresponding Author: Surya Sankar, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy Kerala Veterinary and Animal Sciences University, Pookode, Wayanad-673 576, Kerala, India. Email: surya@kvasu.ac.in

Online published on 17 February, 2020.

Abstract

A total of thirty milk samples from bovine clinical mastitis are subjected to cultural isolation followed by antibiogram of the isolates. All the isolates obtained were resistant to methicillin and penicillin and most of them showed resistance to other beta lactam group of antibiotics. Out of the thirteen Staphylococcus spp. isolated, ten were coagulase negative and all showed positive amplicons for the presence of blaZ gene in polymerase chain reaction. Ten isolates showed positive results for the presence of mecA gene.

Keywords

Bovine mastitis, blaZ, mecA, PCR