1Laboratory of Veterinary Pharmacology and Toxicology, College of Animal Science and Veterinary Medicine, Xinyang Agriculture and Forestry University, Xinyang, 464 000, PR China
2College of Animal Science and Technology, North West Agricultural and Forestry University, Yangling, Shanxi, 712 100, PR China
*Corresponding Author: Zhaowei Ye, Laboratory of Veterinary Pharmacology and Toxicology, College of Animal Science and Veterinary Medicine, Xinyang Agriculture and Forestry University, Xinyang, 464 000, PR China, Email: 953355126@qq.com
Online published on 30 October, 2020.
Bacillus subtilis strains were constructed that overexpressed the glutamyl t-RNA reductase (HemA) and glutamyl t-RNA synthase (HemL). The target proteins accounted for 11 and 13% of the total soluble protein in maltose induced extracts, respectively. The culture media from these strains was red with wavelength maxima near 400 nm indicating elevated levels of porphyrins. The 5- aminolevulinic acid content for these strains reached 42.57 and 39.78 mg/L after 24 h of induced growth and 65.23 and 68.45 mg/L, respectively, after 60 h. Therefore, hemA and hemL overexpression enhanced the biosynthesis of 5-aminolevulinic acid and these strains can be used for its purification.
Bacillus subtilis, Expression vector, Glutamyl-tRNA reductase gene, Glutamyl-tRNA synthesis gene, 5-aminolevulinic acid
