Detection of genetic polymorphism of ANGPTL4 and POMC gene and correlation with growth traits in Guanling cattle

In this study, the polymorphism of ANGPTL4 and POMC gene in 92 individuals of Guanling cattle was detected using DNA sequencing technology and the correlation between polymorphic loci and growth traits of Guanling cattle was analyzed. Three mutation sites including 387 C > T, 418 C > T and 449 A > T were detected in ANGPTL4 gene. Five mutation sites including 138 C > T, 430 C > T, 644 C > T, 668C > T and 693A >G were detected in POMC gene. The results showed that three polymorphic mutation sites of ANGPTL4 gene in the Hardy - Weinberg disequilibrium (HWE) (P < 0.05), POMC gene in 430 C > T mutation the mutation in HWE equilibrium (P > 0.05), multi peptide information content of ANGPTL4, POMC gene polymorphisms (PIC) showed moderate polymorphism. The correlation analysis results showed that the ANGPTL4 gene 418 C > T mutation genotypes and Guanling yellow body height, body length, chest circumference, abdominal circumference showed significant differences (P < 0.01), 449 A>T mutation genotypes and Guanling yellow body height showed a significant difference (P < 0.05) and chest circumference, abdominal circumference showed significant differences (P < 0.01). POMC gene 644 C > T and 668C > T mutation loci and body length of Guanling yellow cattle showed significant difference (P < 0.05).