Indian Journal of Animal Research

SCOPUSWeb of Science
  • Year: 2020
  • Volume: 54
  • Issue: 11

Immunohistochemical detection of gremlin-2 protein in hen ovarian prehierarchical follicle cells

  • Author:
  • T.L. Tyasi, N. Qin, X. Niu, X. Chen, H. Zhu, X. Sun, F. Zhang, R. Xu
  • Total Page Count: 3
  • Page Number: 1347 to 1349

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Xincheng Avenue 2888, Changchun City of Jilin Province, 130118, P. R. China

*Corresponding Author: R. Xu, Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Xincheng Avenue 2888, Changchun City of Jilin Province, 130118, P. R. China, Email: poultryxu@jlau.edu.cn

Online published on 1 December, 2020.

Abstract

The objective of this work was to investigate the distribution of the gremlin-2 (GREM2) protein in various cells of chicken ovarian prehierarchical follicles (6-8 mm in diameter). Chickens (n=10) were slaughtered at the age of 21 weeks and ovarian prehierarchical follicles were removed from the hen ovary. An immunohistochemical analysis was performed to examine the localization of GREM2 protein and the results showed that GREM2 protein is distributed in the chicken prehierarchical ovarian follicle cells and the expression varied with the cell types. GREM2 protein was more dominant in granulosa cells (GCs) and oocytes (OC) (P<0.05) than other cells in the chicken prehierarchical ovarian follicles. The immunohistochemical study suggests that GREM2 protein might play an important role in GCs proliferation and OC maturation of chicken prehierarchical ovarian follicles during growth and development.

Keywords

Gremlin-2, Immunohistochemistry, Prehierarchical follicle, Protein