Department of AGB, College of Veterinary Science and A. H., Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India
* Corresponding Author: Akhilesh Pandey, Department of AGB, College of Veterinary Science and A. H., Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India, Email: akpandey1109@rediffmail.com
Online published on 30 June, 2020.
Research work was carried out on 50 Malvi and 50 Nimari breed of cattle in the Department of Animal Genetics and Breeding of College of Veterinary Science and A.H Jabalpur. In present research work the PCR amplified products of 121bp was digested by restriction endonuclease enzyme DdeI, which recognizes G^AATTC sites. Present study showed that the presence of no restriction sites for the enzyme DdeI and only one band of 121bp was observed on the gel and such genotype was designated as A2A2 type. The β- casein gene showed A2A2 genotypes were observed in Malvi and Nimari breeds of cattle. The result of RFLP revealed that the gene and genotypic frequencies of ?-casein (CSN2) gene for A2A2 was 1.00 for both Malvi and Nimari breed of cattle. So 100% frequency of A2 allele was observed in both the breeds of cattle under the study.
Malvi, Nimari, Polymorphism, Beta (β) Casein Gene
