1Department of Veterinary Pharmacology and Toxicology, PGIVER, RAJUVAS, Bikaner-334 001, Rajasthan, India
2Department of Veterinary Pharmacology, COVS, NDVSU, Jabalpur-482 004, Madhya Pradesh, India
3Department of Veterinary Gynecology, PGIVER, RAJUVAS, Bikaner-334 001, Rajasthan, India
*Corresponding Author: N.S. Meena, Department of Veterinary Pharmacology, COVS, NDVSU, Jabalpur-482 004, Madhya Pradesh, India, Email: nmeena2126@gmail.com
Online published on 20 July, 2020.
The present study was undertaken to determine the residues of norfloxacin in muscle, liver and kidney of broiler chicken. On the basis of surveillance study, commonly used antibiotic norfloxacin was selected for quantifying the concentration in broiler chicken meat samples. The HPLC system was equipped with photodiode array and C18 hypersil column. The mobile phase consisting of 1 ml Ortho phosphoric acid (85 per cent v/v), 100 ml water HPLC grade with acitonitrile HPLC grade, 87:12 (v/v) then pH was adjusted to 2.0 with triethylamine HPLC Grade. The results indicated that out of 180 samples analysed, 3 liver samples of target stations, viz. T3, T6 and T10 were found positive for norfloxacin residues with concentrations of 2.00, 2.60 and 2.31 μg/kg, respectively. Samples collected from other target stations did not show residual concentration of norfloxacin in muscle, liver and kidney samples.
Antibiotic, Hypersil column, Norfloxacin, Photodiode array
