11Department of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Saudi Arabia
2Medicinal, Aromatic and Poisonous Plants Research Center (MAPRC), College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Saudi Arabia
3Department of Phytochemistry, National Research Centre, 33 EI Bohouth St., 12622, Dokki, Giza, Egypt
4Department of Chemistry, Government College Ara Khel, FR Kohat, KPK, Pakistan
5Department of Chemistry, College of Science and Arts Sajir, Shaqra University, Kingdom of Saudi Arabia
6Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, Egypt
*Corresponding Author: Abdelaaty A. Shahat and Riaz Ullah, Department of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Saudi Arabia, Email: ashahat@ksu.edu.sa
Online published on 16 September, 2020.
Diospyros kaki L. (Ebenaceae) fruit and leaves are traditionally used for the treatment of hypertension and angina, as well as for their antithrombotic and anti-inflammatory effects. We investigated the anti-inflammatory, analgesic and antipyretic activities of Diospyros kaki L. (Ebenaceae) in order to scientifically validate its pharmacological importance. In the current study, the anti-inflammatory, analgesic and antipyretic activities of ethyl acetate (Per-1), n-butanol (Per-2) and aqueous (Per-3) fractions of a methanol extract of Persimmon leaves were tested. Wistar rats weighing 190–210 g (for the anti-inflammatory assay) and male albino mice (Swiss) weighing 18–24 g (for the antipyretic and analgesic assays) were utilized as the experimental animals. The rats were separated into eight groups (for anti-inflammatory) and seven group (for antipyretic), each consisting of six rats. Each sample was treated orally and two doses: 100 and 200 mg/kg body weight were used in these experiments. The Per-3 sample at a dose of 200 mg/kg produced the highest inflammation inhibition at 59.28%. The same sample at a dose of 200 mg/kg lowered body temperature from 38°C of hyperthermia mice back to 36.65 ± 0.12°C after 120 min, close to the normal temperature (35.21 ± 0.29°C). In the tail flick model, sample Per-3 showed 67.74% highest nociception inhibition. Similar results were obtained with Acetic acid and hot-plate assay. These results confirm that the n-butanol and aqueous fractions of persimmon are active and may be used for further pharmacological screening.
Animal models, Carrageenan, Diospyros kaki L, Pharmacology
