1Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan-843 41, Utah, USA.
2Department of Microbiology and Immunology and Department of Otolaryngology, New York Medical College and School of Medicine, Valhalla-105 95, New York, USA.
Academy of Chinese Medicine Science, Henan University of Chinese Medicine, Zhengzhou-450 000, Henan, China.
*Corresponding Author: Xiu-min Li, Department of Microbiology and Immunology and Department of Otolaryngology, New York Medical College and School of Medicine, Valhalla-105 95, New York, USA. Email: xiu-min.li@outlook.com
Online published on 16 November, 2021.
Golden Syrian hamster (GSH) have many advantages as animal models in preclinical research, but their application is currently limited by the lack of standardized techniques for analyzing gene expression. Quantitative real-time polymerase chain reaction (RT-qPCR) is a sensitive method for analyzing gene expression, but its reliability depends upon the selection of stable reference (“housekeeping”) genes for proper normalization. The current study was aimed to RT-qPCR for investigated to determine the stability housekeeping gene in golden Syrian hamster.
During the period of June 2019 to October 2019, the expression stability of eight commonly-used housekeeping genes(glyceraldehyde-3phosphate dehydrogenase, Gapdh; b-actin, Actb; hypoxanthine phosphoribosyl transferase 1, Hprt1; ribosomal protein L13a, Rpl13a; ribosomal protein S18, Rps18; Beta-2-microglobulin, B2m; Tubulin beta class I, Tubb; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta, Ywhaz) were investigated in lung, liver, spleen and pancreatic tissue of golden Syrian hamsters using Bestkeeper, geNorm, NormFinder software, comparative delta Ct method and RefFinder.
It was found that the stability of gene expression varied among tissue where in Actb was the most stably expressed housekeeping gene for lung tissue, Hprt1 for liver, Rpl13a for spleen and Tubb for pancreatic tissue. In contrast, the least stable housekeeping gene in both liver and spleen was Gapdh, Rps18 in pancreas and Ywhaz in lung tissue. These data provide a critical evaluation of housekeeping genes in GSH tissues that can be used as a guide for selection of the appropriate genes in future studies.
Golden syrian hamster, Housekeeping gene, RT-qPCR
