Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2021
  • Volume: 55
  • Issue: 4

Single nucleotide polymorphism of CDC37, AHSA1 and STIP1 gene in three cattle breeds using SNaPshot technology

  • Author:
  • Xiao Wang1,2, E Guang-Xin1, Ri-Su Na1, Cheng-Li Liu1, Ze-Hui Guo1, Shu-Zhu Cheng1, Bai-Gao Yang1, Yong-Fu Huang1,*
  • Total Page Count: 6
  • Page Number: 378 to 383

1College of Animal Science and Technology, Chongqing Key Laboratory of Forage and Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, Southwest University, Chongqing, 400716, China

2Animal Husbandry and Veterinary Center, Kongtong District, Gansu Province, Gansu Kongtong, 744000, China

*Corresponding Author: Yong-Fu Huang, College of Animal Science and Technology, Southwest University, Chongqing, 400715, China, Email: h67738337@swu.edu.cn

Online published on 20 August, 2024.

Abstract

Heat stress in domestic animals has become a major limit factor for livestock production in tropical and subtropical regions. These traits that adapt to torrid environment are important in livestock breeding.

In this study, we identified and genotyped six Single nucleotide polymorphism for the CDC37, AHSA1 and STIP1 gene in the Droughtmaster, Angus and Simmental cattle breeds using the SNaPshot Multiplex system.

Result of pair-wise differences (FST) revealed three SNP locus (CDC37-6 (A16154247/G), CDC37-7 (C16157867/G), AHSA1- 10 (G89722567/A) were significantly different in the Droughtmaster population than Angus and Simmental cattle breeds. Moreover, it was found that CDC37-7 (C16157867/G) locus deviated from Hardy-Weinberg equilibrium, suggesting that the CDC37-7 (C16157867/ G) genes have been influenced by the selective breeding of the Droughtmaster breed. Therefore, CDC37-7 (C16157867/G) locus could be used as a marker-assisted candidate gene locus of heat tolerance. This study provides valuable information, concerning marker-assisted selection in the breeding of heat stress resistant cattle.

Keywords

Cattle, Heat tolerance traits, SNaPshot