1Department of Veterinary Medicine, College of Veterinary Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar-263 145, Uttarakhand, India
2Department of Pharmacology and Toxicology, College of Veterinary Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar-263 145, Uttarakhand, India
3Department of Surgery and Radiology, College of Veterinary Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar-263 145, Uttarakhand, India
4Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India
*Corresponding Author: Neeraj Thakur, Department of Veterinary Clinical Complex, Faculty of Veterinary and Animal Sciences, Rajiv Gandhi South Campus-Banaras Hindu University, Barkachha, Mirzapur-231 001, Uttar Pradesh, India, Email: niju.thakur92@gmail.com
Online Published on 15 July, 2022.
Acute kidney injury causes an abrupt decline in renal filtration and affects animals in a similar way to humans. Diagnosis can be made based on urinalysis, serum biochemistry and various biomarkers. The present study was conducted to evaluate the ameliorative, antioxidant and immunomodulatory potential of vitamin D in rats induced with acute kidney injury.
In the present study, group A rats were taken as healthy control, group B rats were given gentamicin @ 100 mg/kg BW intraperitoneally for 8 days and were considered as disease control and group C rats were treated with Vitamin D @ 0.4 |jg/kg/day subcutaneously for 8 days along with intraperitoneal gentamicin injection. Reduced glutathione (GSH), lipid peroxide (LPO), catalase and superoxide dismutase (SOD) were estimated in erythrocytes on day 0, 4 and 8. Tumor necrosis factor alpha (TNF a) and interleukin 10 (IL 10) were also estimated along with urine and serum biochemistry on day 0, 4 and 8. Kidney tissue samples were collected on day 8 for histopathological examination.
The mean values of GSH, catalase and SOD were significantly (P<0.05) higher whereas the mean value of LPO was significantly (P<0.05) lower in group C compared to group B on day 4 and 8. On day 4 and 8, the mean value of TNF a was significantly (P<0.05) lower, while the mean value of IL-10 was significantly (P<0.05) higher in rats treated with vitamin D as compared to disease control. Histopathological examination along with urine and serum biochemistry revealed protective efficacy of vitamin D in acute kidney injury. Based on the findings of the present study, it is concluded that vitamin D is having ameliorative efficacy along with antioxidant and immunomodulatory potential in case of gentamicin induced acute kidney injury in Wistar rats. However, detailed studies are required to explore the therapeutic potential of vitamin D in clinical cases of kidney diseases.
Acute kidney injury, Gentamicin, IL-10, TNF-a, Vitamin D
