1Department of Veterinary Surgery, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria
2Department of Veterinary Anatomy, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria
*Corresponding Author: N.H. Okereke, Department of Veterinary Surgery, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria, Email: nnamdi.okereke@unn.edu.ng
Online Published on 15 July, 2022.
Mean values of erythrocytic morphometric parameters of very old blood and its effect on the target post-transfusion haematocrit changes of splenectomised dogs was studied.
Two hundred and fifty milliliters of blood each were drawn from healthy dogs (n=6) into citrate phosphate dextrose adenine-1 anti-coagulated blood bags, preserved for 35 days for the evaluation of erythrocyte morphometric and viability parameters. Thereafter, twenty adult male splenectomised dogs were randomly assigned into 5 groups (n=3). Post-splenectomy, 4, 14, 21 and 28 day old blood (DOB) were transfused to groups II-V while group I animals were not transfused. Intraoperative blood loss was determined during the surgery while post-transfusion, animals haematocrit were assayed and used to calculate the targeted haematocrit.
Findings revealed irreversible progressive time dependent morphometric changes by day 14 of blood storage. Hence, it is recommended that for transfusion purposes, 4 DOB should be the hallmark as it achieved the desired haematocrit and no morphometric changes were observed from it.
CPDA-1, Erythrocyte, Hematocrit, Morphometric, Trypan blue
