1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia.
2Study Program of Animal Husbandry, Faculty of Agricultural and Animal Sciences, Universitas Islam Kebangsaan, Bireuen24251, Indonesia
3Agency for Fisheries Affair of Aceh Tengah District, Takengon, Indonesia
4Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda, Indonesia
5Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh23111, Indonesia
6Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh23111, Indonesia
*Corresponding Author: Kartini Eriani, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh23111, Indonesia. Email: kartini_eriani@unsyiah.ac.id
Online published on 1 August, 2022.
Sperm cells are susceptible to oxidative stress during cryopreservation. Therefore, an antioxidant is necessary to protect them from damages. Fetal bovine serum (FBS) is one of potent antioxidants for fish sperm cryopreservation. Hence, the aims of this study are to examine the effect of FBS on sperm quality after a short period and to determine its optimum concentration on depik (Rasbora tawarensis).
Depik fish were obtained from the Fish hatchery of Lukup Badak, Aceh Tengah District, Indonesia. Sperms collected from the fish were diluted in Ringer extenders containing FBS concentration of 10% (P1), 20% (P2), 30% (P3), 40% (P4), 50% (P5) and 60% (P6), filled into 2 ml cryotubes and equilibrated prior immersed into liquid nitrogen for 15 days. The parameters observed were sperm motility, consistency, pH, fertilization and hatching rates and DNA fragmentation post-thawing.
The ANOVA test indicates that the application of FBS in Ringer had a significant effect on sperm motility, fertilization and hatching rates (P<0.05). The highest motility (58.33%) was recorded at FBS 60% and significantly different from those at other concentrations. The laddering analysis showed that applying FBS protected the integrity of depik sperms DNA. It is concluded that the optimum concentration of FBS on depik sperm led to a short-term cryopreservation of 60%.
Cryopreservation, Depik fish, DNA integrity, Fetal bovine serum, Hatching rate
