1Khalsa College of Veterinary and Animals Sciences, Amritsar-143 002, Punjab, India
2Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 001, Punjab, India
3Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 001, Haryana, India
*Corresponding Author: Harmeet Singh Sandhu, Khalsa College of Veterinary and Animals Sciences, Amritsar-143 002, Punjab, India. Email: harmeetsinghsandhu29@gmail.com
Online published on 17 March, 2023.
Standard estrus synchronization regimens resulted in variable outcomes in buffaloes. The present study evaluated ovarian and fertility responses following presynchronization in Heatsynch protocol administered in postpartum buffaloes.
In group-I (Presynch-Heatsynch group, n=30), PGF2α was administered on days -14 and -2. Then, GnRH analogue, PGF2α (Cloprostenol) and Estradiol benzoate were administered on day 0, 7 and 8, respectively followed by fixed-time artificial insemination (FTAI) 48 hours later. In group-II (Heatsynch group, n=30), rest protocol was same, except that first two PGF2α injections were not administered.
The progesterone concentrations differed (P<0.01) between the two groups on days -2, 0 and 7. Post-treatment, progesterone profiles were also higher in pregnant compared to non-pregnant buffaloes in both the groups except on day 10. CL diameter differed (P<0.01) between groups on days -2, 0 and 7. It was larger in pregnant than non-pregnant buffaloes on day 7 in both the groups. Dominant follicle diameter remained larger on days -2, 0 and 8 in group-I than -II. Buffaloes getting pregnant had a larger (P<0.01) dominant follicle size on the day of FTAI in group-I than of group-II. Ovulatory response of 93.33 and 90.00% was observed in group-I and II. The conception rate was higher (66.66 vs. 40.00%; P<0.05) in group-I than Group-II. Presynchronization improved reproductive efficiency in Heatsynch treatment and may aid for better fertility in buffaloes.
Buffalo, Estrus synchronization, Fertility, Heatsynch, Presynchronization
