1Department of Stomatology, The General Hospital of Western Theater Command, Chengdu, 610038, Sichuan, People's Republic of China
*Corresponding Author: Shuyong Yang, Department of Stomatology, The General Hospital of Western Theater Command, Chengdu, 610038, Sichuan, People's Republic of China, Email: 13558702367@163.com
Online published on 4 April, 2023.
MicroRNA-143 (miR-143) has been reported that its biological function is to serve as a target to inhibit tumor progression in human oral squamous cell carcinoma (OSCC). However, its role and molecular mechanisms in tongue squamous cell carcinoma (TSCC) were still unknown.
Several in vitro experiments were performed using CAL-27 cells, including cell proliferation experiment, wound healing experiment, flow cytometry, transwell and colony formation experiments. In addition, we also performed in vivo experiments to study the potential application of miR-143 in tumorigenesis of TSCC.
The result exhibited that miR-143 up-regulation could significantly suppress proliferation, migration and invasion ability of CAL-27 cells and make these cells stop at G0/G1 phase. We reported that upregulation of miR-143 would suppress its tumorigenic ability. Moreover, we also found that the overexpression of miR-143 would reduce the phosphorylation level of STAT3, p65 and AKT. In addition, miR-143 overexpression decreases CDK2 expression while as expression level of E-cadherin was increased.
AKT/STAT3/NF-κB, miR-143, Tongue squamous cell carcinoma, Tumor progression
