1Division of Animal Genetics and Breeding, ICAR-National Dairy Research Institute, Karnal-132 001, Haryana, India
*Corresponding Author: Oshin Togla, Division of Animal Genetics and Breeding, ICAR-National Dairy Research Institute, Karnal-132 001, Haryana, India, Email: Toglaoshin2121@gmail.com
Online published on 17 June, 2025.
The experiment aimed to investigate polymorphisms in exon 2 of the PHKA2 gene and investigate the relationship between identified single nucleotide polymorphism and udder type traits in Sahiwal cows. Udder morphometry is being used as a forecaster of production performance in cows since older times. Finding the variants associated with these traits in largely variable region of PHKA2 gene can prove to be highly beneficial.
DNA isolated from Sahiwal cows was analysed by DNA sequencing. Nine udder type and five teat types were measured for each animal according to procedure followed by International committee of animal recording (2012).
Three SNPs g.124497381C>T, X: 124497248 G>A and X: 124497189 C>T were identified. Recessive homozygotes were negligible as mutant allele exhibited very low ranging frequency (from 0.02 to 0.08) for the targeted loci. Identified point mutation g. 124497381 C>T was found to be significantly (p<0.05) associated with distance between teats and central ligament, SNP X: 124497248G>A with rear udder width, fore udder attachment, udder depth, udder length and teat length (p<0.05) and SNP X: 124497189 C>T with udder length and rear udder height (p<0.05). Interaction of identified SNPs and udder traits highlighted the gene's potential as a candidate gene for selecting for conformation traits in Indian Sahiwal cattle.
Association, Exon: II, PHKA2 gene, Polymorphism, Sahiwal cattle, Udder type traits
