1School of Animal Life Convergence Science, Hankyong National University, Anseong, 17579, South Korea
2Department of Animal Husbandry, Faculty of Agriculture, University of Jember, Jember, 68122, Indonesia
3Department of Basic Veterinary Sciences, College of Veterinary Medicine, University of the Philippines Los Banos, Laguna, 4031, Philippines
4Research Council of SoRegen Convergence Science, Seoul, Republic of Korea
Peripheral blood mononuclear cells (PBMCs) represent an attractive tissue source in pharmacogenomics and molecular and immunologic studies. In order to investigate the changes in biological effects, water samples were exposed to a specific quantum entanglement signal from the SoRegen chip for 48 hours. This study aimed to evaluate the immunomodulatory and anti-inflammatory effects of SoRegen Technology in broiler and layer hens PBMCs.
Six-week-old Arbor Acress broiler chickens and 66-week-old layer hens Lohmann were used for the establishment of an
The result indicates that post-treated water media enhanced the proliferative properties of broiler and layer hens PBMCs. The mRNA and protein expression of immune-modulating and pro-inflammatory cytokines: IL-2, IL-6, iNOS, IFNγ, TNFα and NFκB exhibited a marked increase following stimulation of the cells by LPS and Con A when compared with the non-stimulated cells. However, stimulated cells grown in structured water media showed a strikingly decreased expression of pro-inflammatory cytokines, except for the upregulation of IFNγ expression in broiler PBMCs but not in layer hens PBMCs. It can be concluded that SoRegen® Technology water had anti-inflammatory activities with potential clinical immunomodulatory effects in younger chicks.
Anti-inflammatory, Immunology, PBMCs, SoRegen®, Technology
