Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2024
  • Volume: 58
  • Issue: 2

Immunomodulatory effect of Eugenia caryophyllata against vitamin D-induced toxicity in liver of male westar rats

  • Author:
  • Manal F. El-Khadragy1, Noura M. Al-Durgham2, Sherifa S. Hamed3, Fatma Elzahraa H. Salem4, Maha A. Aljumaa1, Hany M. Yehia5,6, Mariam A. Alkhateeb1,*
  • Total Page Count: 10
  • Page Number: 200 to 209

1Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia

2Department of Zoology, College of Science, King Saud University, P.O. 2455, Riyadh, 11451, Saudi Arabia

3Department of Zoology Faculty of Science, Alexandria University, Alexandria, Egypt

4Department of Zoology and Entomology Faculty of Science, Helwan University, Cairo, Egypt, P.O. Box 11611, Egypt

5Department of Food Science and Nutrition, College of Food and Agriculture Science, King Saud University, Riyadh, 1145, Saudi Arabia

6Department of Food Science and Nutrition Faculty of Home Economics, Helwan University, Helwan, 11111, Egypt

*Corresponding Author: Mariam A. Alkhateeb, Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia, Email: maaikhateeb@pnu.edu.sa

Online published on 9 August, 2024.

Abstract

Vitamin D is essential for overall health, wellbeing and immune system. hypervitaminosis D led to many deleterious effects and increased mortality. This study aimed evaluate the immunomodulatory potential of eugenol (volatile phenolic constituent of clove essential oil obtained from Eugenia caryophyllata) against inflammation induced by vitamin D toxicity.

Vitamin D (100 mg/kg) was orally administered into male albino rats followed by an oral administration of eugenol (100 mg/kg) for two months.

Histological results showed Vitamin D intoxication cause distinct alterations such as loss of lobular architecture, fibrosis and necrosis, treatment of eugenol with injected of Vitamin D showed improvement in the histological features of liver tissues. The study showed that a significant upregulation in the TNF-α, IL-2 and iNOS genes expression levels caused by Vitamin D compared to the control group. Eugenol treatment caused a significant decreased TNF-α, IL-2 and iNOS activity and down-regulated gene expression levels. In Conclusion, hepatoprotective effects of eugenol through inhibiting pro-inflammation by vitamin D toxicity and increase the activity of the antioxidant defense system.

Keywords

Antioxidants, Clove, Gene expression, Inflammation, Liver, Vitamin D