Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2024
  • Volume: 58
  • Issue: 3

Genetic polymorphism of growth hormone gene in dorper sheep using PCR-RFLP

  • Author:
  • Molabe Kagisho Madikadike1, Thobela Louis Tyasi1,*, Vusi Mbazima2, Busisiwe Gunya1
  • Total Page Count: 5
  • Page Number: 371 to 375

1Department of Agricultural Economics and Animal Production, School of Agricultural and Environmental Sciences, University of Limpopo, Private Bag X1106, Sovenga, 0727, Limpopo, South Africa

2Department of Biochemistry Microbiology and Biotechnology, School of Molecular and Life Sciences, Private Bag X1106, Sovenga, 0727, Limpopo, South Africa

*Corresponding Author: Thobela Louis Tyasi, Department of Agricultural Economics and Animal Production, School of Agricultural and Environmental Sciences, University of Limpopo, Private Bag X1106, Sovenga, 0727, Limpopo, South Africa, Email: louis.tyasi@ul.ac.za

Online published on 9 August, 2024.

Abstract

The growth hormone gene has a significant impact on postnatal longitudinal growth and development as well as the metabolism of protein, carbohydrates, and fats moreover its effects indirectly relate to bone and skeletal development. The objective of the study was to identify a single nucleotide polymorphism of the growth hormone gene and its association with growth traits in Dorper sheep.

Data were collected from 50 Dorper sheep of 1 to 2 years of age at the University of Limpopo Experimental farm. The objective of the study was achieved by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and General Linear Model (GLM).

PCR-RFLP reports revealed different segment patterns resulting in two genotypes such as AA and AB genotypes. Marker-traits association analysis showed that no significant difference was observed between AA and AB genotypes regarding all the measured traits except with wither height and AA genotype was remarkably related to higher withers height than AB genotype. It can be concluded that GH1 could be used as a genetic marker for marker-assisted selection (MAS) during breeding for the improvement of withers height.

Keywords

Exon 4, Genetic selection, Marker-trait association, Restriction fragment length polymorphism