Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2024
  • Volume: 58
  • Issue: 4

The transcriptional landscape of the early embryonic developmental biology in hulunbuir short-tailed sheep

  • Author:
  • Hong Su1,3, Lu Chen2,3, Guang Yang1,3, Da-Qing Wang4, Xiu-Nan Li4, Yong-Li Song5,*, Gui-Fang Cao1,2,3,**
  • Total Page Count: 7
  • Page Number: 566 to 572

1College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia-010010, China

2Inner Mongolia Autonomous Region Key Laboratory of Basic Veterinar Medicine, Huhhot, Inner Mongolia-010010, China

3Animal Embryo and Developmental Engineering Key Laboratory of Higher EducationInstitutions of Inner Mongolia Autonomous Region, Huhhot, Inner Mongolia-010010, China

4Inner Mongolia Academy of Agriculture and Animal Husbandry, Huhhot, Inner Mongolia-010031, China

5College of Life Sciences, Inner Mongolia University, Huhhot, Inner Mongolia-010021, China

*Corresponding Author: Yong-Li Song, College of Life Sciences, Inner Mongolia University, Huhhot, Inner Mongolia-010021, China, Email: songyongli625@163.com

**Gui-Fang Caom, College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia-010010, China, guifangcao@126.com

Online published on 13 August, 2024.

Abstract

Since both fetal and postnatal development is determined by early embryonic development and the molecular mechanism and organogenesis process of early mouse embryos have been thoroughly studied. However, the specific development process of early embryos in Mongolian sheep remains poorly understood.

The 16-day and 25-day embryo samples of Inner Mongolia Hulunbuir short-tailed sheep were evaluated by transcriptome sequencing technique and the genes with significant differences were screened and the gene function was enriched by gene ontology (GO) database and Kyoto Encyclopedia of Gene and Genome (KEGG) database.

A total of 4348 differentially expressed genes (DEGs) were analyzed by sequencing and especially significant DEGs included the genes such as COL6A2, COL4A4, LAMA4, ITGA8, FN1 involved in ECM-receptor interaction, focal adhesion, PI3K-Akt signaling and protein digestion and absorption pathways; YAP1, FZD4, TGFB1, WNT2, BMP5, BMP2, WNT11 involved in Hippo signaling pathway. In pathway analysis, those pathways mainly related to cell interaction and organogenesis were highlighted at the E16 stage. They are critical for transcriptional control in early embryonic development, especially during organ building. The genes that make organs and body structures more complex and detailed and those related to the development of brain, liver and immune system are enriched for the E25 stage. Therefore, this study provides important supplementary and exploratory reference data for the further research on early embryonic development in sheep.

Keywords

Embryo of hulunbuir short-tailed sheep, Early embryonic developmental, Organogenesis, RNA-seq