1Department of Veterinary Microbiology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Navania, Udaipur-313 601, Rajasthan, India
2Department of Veterinary Public Health and Epidemiology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Navania, Udaipur-313 601, Rajasthan, India
*Corresponding Author: Rajesh Singathia, Department of Veterinary Microbiology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Navania, Udaipur-313 601, Rajasthan, India, Email: drrajeshsingathia@gmail.com
Online published on 13 August, 2024.
Subclinical mastitis is one of the most important diseases of economic importance affecting dairy animals worldwide. The present study was planned to evaluate the level of expression of toll-like receptors, cytokines and acute phase protein in milk somatic cells during subclinical mastitis of cattle.
The milk samples of subclinical mastitis-positive and healthy cattle were collected. After that, extraction of total RNA was done from milk somatic cells followed by cDNA synthesis by the process of reverse transcription. Following that qPCR was carried out and relative transcript levels were determined.
In the present study, a relative up-regulated expression was found in TLR-2, IL-1β, IL-10 and Hp and down-regulation was found in TLR-4, TNF-α, IFN-γ and IL-6 in the milk of cattle with subclinical mastitis as compared to healthy ones. Monitoring of cytokines entangled in the modulation of immune responses during the infection is useful in deciding cytokine markers that could be employed as a forecasting tool in the early diagnosis of subclinical mastitis.
Acute phase protein, Cytokine, Subclinical mastitis, Toll-like receptors
