Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2025
  • Volume: 59
  • Issue: 11

Effects of Frozen Semen Extender for Goats (MJ Ex2™ and Tris-egg Yolk) on Sperm Motility Quality after Freezing

  • Author:
  • Nittaya Thongtip1, Wiwat Pattanawong2, Patcharee Promtan3, Paweenisaras Khenjan3, Piyamas Tancharoenrat1, Watee Kongbuntad4,*
  • Total Page Count: 7
  • Page Number: 1834 to 1840

1Faculty of Science and Agricultural Technology, Rajamangala University of Technology, Lanna, Lampang-52000, Thailand

2Faculty of Animal Science and Technology, Maejo University, Chiang Mai-50290, Thailand

3Faculty of Agriculture and Natural Resources, Rajamangala University of Technology, Tawan-ok, SrirachaChonburi-20110, Thailand

4Faculty of Science, Maejo University, Chiang Mai-50290, Thailand

*Corresponding Author: Watee Kongbuntad, Faculty of Science, Maejo University, Chiang Mai-50290, Thailand, Email: wateenaka@gmail.com

Online published on 23 January, 2026.

Abstract

Semen extenders are crucially important when freezing animal semen to protect against sperm cell death and preserve the quality of the sperm cells. Semen diluents play a crucial role in the production process of freezing semen. Both pre-and post-freezing methods can be employed until the point of insemination.

This study aimed to compare the quality of sperm motility after freezing a newly produced semen Extender (MJ Ex2™) with a conventional formula (Tris-egg yolk) using Computer Assisted Sperm Analysis (CASA). This was achieved by dividing the semen into two portions, diluting each portion with a different formula and then freezing both semen specimens using the same process.

The results indicate that the fresh goat semen samples in this study had a cream color, an average volume of 1.10±0.87 ml/ time, a density of 4D (thick), a viscosity of 4°C (milky), a pH of 6.53±0.14, a concentration of 17,203±275.18 ×106/ml and a motility of ++++ (75.7±0.13). The MJ Ex2™ extender diluent had a movement percentage of 76.72±5.00, which was significantly higher than the Tris-egg yolk formula of 66.48±2.89 (P<0.01). As for the percentage of forward movement, VAP, VSP, ALH and BCF values were not different. The motility characteristics of the Tris-egg Yolk formula had a LIN value of 50.19±4.05, which was significantly high (P<0.01). The percentage of motility and STR values of sperm diluted with MJ Ex2™ extender was 78.14±5.04, which was significantly higher than the Tris-egg yolk formula 72.60±7.93 (P<0.05). The results of this study indicate that the MJ Ex2™ extender formula enhanced the quality of frozen goat semen. Considering the motility of the sperm; however, would suggest that other effects on those sperm cells would need to be furthers to investigate.

Keywords

Extender, Goat artificial insemination, Goat semen, Sperm motility