Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2025
  • Volume: 59
  • Issue: 11

Detection and Isolation of Peste-des-petits Ruminants Virus (PPRV) Infection in Sheep and Goats

  • Author:
  • Shalini Pandey1,*, Gurpreet Kaur1, P.N. Dwivedi1
  • Total Page Count: 3
  • Page Number: 1957 to 1959

1Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India

*Corresponding Author: Shalini Pandey, Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India, Email: dr.shalini9651@gmail.com

Online published on 23 January, 2026.

Abstract

PPR also known as goat plague is an acute highly contagious disease of sheep and goats characterized by fever, catarrhal inflammation of ocular and nasal mucous membrane and diarrhea. The present study was conducted for molecular detection and isolation of Peste des petits ruminants (PPR) virus in sheep and goats in Punjab region. A total of 150 samples; ocular (25), nasal (25) and rectal swabs (25) separately from sheep and goats were collected. These samples were subjected to Reverse Transcriptase-PCR for molecular detection of PPR virus. Out of 75 samples from 25 goats, three samples (4%) were found positive for F gene by RT-PCR and all the samples from sheep were negative for PPR virus. PPR virus was isolated from one sample using vero cell line.

Keywords

Goat, PPR, RT-PCR, Sheep, Virus isolation