1Department of Veterinary Anatomy, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, Tamil Nadu, India
2Department of Veterinary Anatomy, Veterinary College and Research Institute, TANUVAS, Theni-625 602, Tamil Nadu, India
3Centre for Stem Cell Research and Regenerative Medicine, Madras Veterinary College, TANUVAS, Chennai-600 007, Tamil Nadu, India
4Department of Veterinary Anatomy, Veterinary College and Research Institute, TANUVAS, Salem-636 112, Tamil Nadu, India
5Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai-600 007, Tamil Nadu, India
*Corresponding Author: Soundian Eswari, Centre for Stem Cell Research and Regenerative Medicine, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, Tamil Nadu, India, Email: drseswari@gmail.com
Online Published on 07 July, 2025.
Mesenchymal stem cells are well known for their self-renewal capacity and ability to differentiate into multiple cell lineages. The aim of the study was to develop a simple technique for isolation of mesenchymal stem cells from porcine adipose tissue and to study the morphometric characteristics of porcine mesenchymal stem cells.
Porcine adipose derived mesenchymal stem cells were isolated in vitro by using collagenase type II enzyme. Cell yield and viability of the cells were calculated by using trypan blue exclusion method using Neubauer’s chamber. Characterization of MSCs were done by using specific cell markers. The morphological changes, morphometry were analysed in culture using Leishman’s stain. The cell doubling (CD) and Population doubling time (PDT) were also calculated.
The isolated adherent cells start forming colony and demonstrated an elongated, round and spindle like fibroblastic morphology by day 1. Almost 80-90 per cent confluency was attained on day 8-9 after the initial seeding and was reduced to day 3-4 in the subsequent passages. RT-PCR reactions revealed positive expression of mesenchymal stem cell markers CD44, CD73 and negative expression of CD34, a hematopoietic cell surface marker. Immunocytochemistry also revealed positive expression for CD44 and negative for CD34. In morphometric studies, the cell length, nucleus length, cell width and nucleus width were increased between 24 and 48 hours in both P2 and P3.
Immunocytochemistry, Morphometry, MSCs, Porcine adipose tissue, RT-PCR
