1Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India.
*Corresponding Author: R. Gangil, Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India. Email: rgangil@gmail.com
The present study was aimed at cloning and expression of the major outer membrane porin (ompH) gene of P. multocida serotype B:2 P52, the strain used in vaccines against HS in India. The Porin H (OmpH) is major immunodominant outer membrane protein in the envelope of Pasteurella multocida.
The gene encoding OmpH was amplified by PCR, cloned and expressed in prokaryotic expression vector. The recombinant OmpH protein was expressed as a protein with E. coli BL21 (DE3) cells transformed with recombinant plasmid pQE30-Xa-ompH.
The expressed protein was purified from E. coli and characterized by SDS-PAGE and western blot analysis. The fusion recombinant protein eluted had a molecular mass of about 34 kDa. The expressed recombinant protein was confirmed with western blot analysis using RGS-His antibody and anti-P. multocida serum raised against whole cell lysate.
Cloning and expression, Haemorrhagic septicaemia, Outer membrane protein, Pasteurella multocida, Recombinant protein
