Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2026
  • Volume: 59
  • Issue: 7

Effect of Leptin and IGF-I Supplementation on in vitro Maturation and Expression ofApoptotic Genes in Goat Oocytes

  • Author:
  • Saleema Ahmedi Quadri13*, Satya Nidhi Shukla1, Ajit Pratap Singh2, Bikash Chandra Sarkhel2
  • Total Page Count: 5
  • Page Number: 1115 to 1119

1Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Jabalpur-482 001, Madhya Pradesh, India.

2Animal Biotechnology Centre, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India.

3Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Mathura-281 001, Uttar Pradesh, India.

*Corresponding Author: Saleema Ahmedi Quadri, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Jabalpur-482 001, Madhya Pradesh, India. Email: saleemaquadri94@gmail.com

Abstract

The quality of gametes is a crucial factor for the successful development of an embryo. The potential of oocyte development is acquired during oogenesis by many changes at the molecular and cellular levels. The present study explores the effect of leptin and IGF-I supplementation on in vitro oocyte maturation and the expression of apoptotic genes in matured goat oocytes.

In vitro maturation of abattoir-derived oocytes was in maturation media having supplements as the group I (control), group II (leptin 20 ng/ml) and group III (IGF-I 100 ng/ml). From these oocytes, RNA was isolated and expressions of apoptotic genes were studied.

There was no significant difference found between cumulus expansion and also in nuclear maturation in groups I, II, III. The relative expression of BAX gene was 0.65 fold lower in group II and 0.42 fold lower in group III as compared to group I whereas relative expression of Mcl-1 gene was 2.55 fold higher in group II and 0.31 fold lower in group III as compared to group I.

Keywords

Apoptotic gene, Goat oocytes, IGF-I, In vitro maturation, Leptin