Spectroscopic Analysis of Copper Minocycline Novel Complex and Evaluation of its Potent Antibacterial, Antioxidant and Anti-breast Cancer (MCF-7) Properties

Minocycline (Mino) has a longer half-life than 1^st-generation tetracycline. A trace element, Cu is necessary for the operation of numerous cellular enzymes as it is an essential component in free radical scavenging and enzyme redox chemistry.

FT-IR, electronic spectra (UV), X-ray diffraction (XRD), thermogravimetric analysis, SEM, TEM were used to characterize the novel complex (Mino/Cu). The molar conductance value validates that the Cu (II) complex is not electrolytic. Mino is chelated with Cu (II) via (C=O) ketonic oxygen atoms and (OH) groups based on spectral data. Cu (II)’s complex surface morphology showed up as tiny rectangular projections, according to SEM analysis. TEM showed that the Cu (II) chelate formed black spots with particle sizes between 9 and 23 nm. Cytotoxicity against MCF-7 breast cancer cell proliferation and antibacterial activity against three resistant strains (Salmonella enteritidis, Klebsiella pneumonia and Bacillus subtilis) are estimated in vitro along with the scavenging capabilities of ORAC free radicals.

The study found that the Mino/Cu complex had strong anticancer properties, inhibiting MCF-7 proliferation growth and cancer cell viability by 57.54 μg/ml at concentrations of 100 ug/ml. It also showed strong antibacterial properties against S.enteritidis, K. pneumonia and B. subtilis at low concentrations. The complex also demonstrated high antioxidant activity with ORAC activity 1581 (μM Trolox eq/ml).