The Potential Influence of Coleus forskolii, Green Coffee and Green Tea on Body Weight, Reproductive Performance, Hematological Profiles in Mice

Coleus forskolii, green coffee and green tea have been shown to effect on body functions and health.

Twenty males and thirty female albino mice of 33.86 ±0.27g BW were classified into two groups; control group fed basal control diet versus treated group fed basal control diet containing multi-ingredient supplement (Coleus forskohlii, green coffee bean and green tea; 1.5% per supplement) for ten weeks. Initial and final body weights were recorded of control and supplemented groups. Germinal vesicle oocytes were harvested from ovaries after 48:0h of PMSG injection for investigating oocyte quality. Furthermore, female mice were mated with fertile males and pregnancy rate and offspring numbers were recorded. In addition, the male mice of both groups were sedated using 26.6 mg/kg BW xylazine for recording rectal temperature, pulse rate and SPO₂. Besides, blood samples were collected for complete blood picture and plasma biochemistry analyses.

The results showed that multi-ingredient supplement caused significant decrease in body weight gain versus increase (P<0.05) of rectal temperature. Reproductive performances represented by oocyte quality and pregnancy rate were significantly decreased in multi-ingredient supplement group. Furthermore, blood cells and plasma profiles (urea, creatinine, glucose, aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase) were decreased in multi-ingredient supplement groups except total protein if compared to control group. It could be concluded that multi-ingredient supplement resulted in significant decrease of body weight gain, reproductive performances and blood cell profiles as well.