1Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Dr. Ambedkar Nagar-Mhow-453 446, Indore, Madhya Pradesh, India.
2Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Dr. Ambedkar Nagar-Mhow-453 446, Indore, Madhya Pradesh, India.
*Corresponding Author: S.D. Audarya, Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Kuthuliya-486 001, Rewa, Madhya Pradesh, India. Email: asd_vet@yahoo.com
Canine parvovirus-2 causes inflammation of the gastrointestinal tract in dogs. Dogs from Mhow and Indore area suffering from symptoms related to gastroenteritis were investigated for the presence of Canine parvovirus-2 infection.
A total of 50 faecal samples from dogs (33 male and 17 female) were collected individually in 5 ml of phosphate buffer saline solution in sterile containers. These samples were tested in haemagglutination assay using pig red blood cells for the presence of Canine parvovirus-2 specific antigen and in a molecular test, polymerase chain reaction, using primers to amplify Canine parvovirus-2 specific product of 681 base pairs.
A total of 5 faecal samples (10%) tested positive in haemagglutination assay indicative of presence of Canine parvoviral-2 antigen in the faecal material. Haemagglutinating titre for positive samples ranged from 32 to 1024. Only one faecal sample found positive in polymerase chain reaction test for amplification of Canine parvovirus-2 specific product. The results of the present study indicate presence of Canine parvoviral-2 infection in pet dogs suffering from gastroenteritis, however at a low level.
Canine parvovirus-2, Faecal samples, Haemagglutination, Polymerase chain reaction
