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*Corresponding Author: Prathisha Rajamani, Chrisolite Bagthasingh;
Nanoparticles have gained attention in animal health for their applications as feed additives, antimicrobials, vaccine adjuvants and immunostimulants. This study aims to explore their potential as an eco-friendly alternative to conventional antibiotics in aquaculture.
In this study, zinc oxide nanoparticles were synthesized from pomegranate rind (P-ZnO NPs), characterized using UV-Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Field Emission Scanning Electron Microscopy with Energy Dispersive X-ray Spectroscopy (FESEM-EDS) and their antibacterial and antioxidant activities were evaluated by well diffusion and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays.
P-ZnO NPs exhibited a spherical morphology with an average size of 84.15 nm, strong antioxidant activity (IC50 = 98.48±0.89 μg/mL) and significant antibacterial effects against Photobacterium damselae subsp. damselae (MIC = 6.25 μg/mL), P. damselae subsp. piscicida and Aeromonas hydrophila (MIC = 3.12 μg/mL). These results highlight pomegranate rind mediated ZnO nanoparticles as a green and effective approach for controlling major aquaculture pathogens.
Antibacterial, Antioxidant, Green synthesis, Nanoparticles, Zinc oxide