Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2026
  • Volume: 60
  • Issue: 1

Multidrug-resistance Pattern of Antibiogram of Escherichia coli and Staphylococcus aureus Isolated from Mastitis Affected Buffaloes in Eastern Uttar Pradesh

  • Author:
  • S.V. Singh1, J.P. Singh1, Vibha Yadav2, Sandeep Kumar Yadav1, Ramakant1*, P.K. Maurya3, R.K. Joshi2
  • Total Page Count: 8
  • Page Number: 100 to 107

1Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and TechnologyKumarganj, Ayodhya-224 229, Uttar Pradesh, India.

2Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and TechnologyKumarganj, Ayodhya-224 229, Uttar Pradesh, India.

3Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224 229, Uttar Pradesh, India.

*Corresponding Author: Ramakant, Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and TechnologyKumarganj, Ayodhya-224 229, Uttar Pradesh, India. Email: ramakant.dr2@gmail.com

Abstract

Mastitis is a problem of dairy animals including buffaloes. Treatment failure has become a common problem and the most cited reason is antibiotic resistance. The paper presents the scenario of antibiotic resistance with special reference to multi drug resistance pattern.

Microbiological assay of mastitis affected 48 milk samples was carried out using standard protocols to establish the prevalence of mastitis caused by E. coli and S. aureus. Antibiotic sensitivity test was carried out against these organisms and the whole milk culture for commonly used antibiotics. The drug resistance pattern was established.

The overall prevalence of mastitis was 20%. The prevalence of E. coli and S. aureus was 29.17% and 54.17% respectively. The antibiotic sensitivity revealed that E.coli isolates were 100% susceptible to tetracycline, gentamicin, enrofloxacin and streptopenicillin followed by Ceftriaxone and sulbactum combination and streptomycin (92.86%) and ceftriaxone and methicillin (85.71%). The isolates of S. aureus were 100% sensitive to only two antibiotics gentamicin and streptopenicillin. The antibiogram of whole milk culture revealed maximum susceptibility to enrofloxacin, gentamicin and streptopeniciliin (95.83% each). Multiple drug resistance has been observed in this study.

Keywords

Antibiogram, Antibiotic sensitivity, Buffalo, Drug resistance pattern, Mastitis