Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2026
  • Volume: 60
  • Issue: 1

Molecular and Cultures based Diagnosis of Infectious Bursal Disease Virus (IBDV) in Vaccinated and Non Vaccinated Poultry Flocks

  • Author:
  • Ritesh S. Shinde1, Harshad C. Chauhan1, Sandip S. Patel1, Kishan Kumar Sharma1*, Arun C. Patel1, Mehul D. Shrimali1, Sushil Kumar Mohapatra1, Bharat Singh Chandel1
  • Total Page Count: 5
  • Page Number: 130 to 134

1Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Banaskantha-385 005, Gujarat, India.

*Corresponding Author: Kishan Kumar Sharma, Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Banaskantha-385 005, Gujarat, India. Email: kishan12sharma@rediffmail.com

Abstract

In recent years, Infectious bursal disease is continuously occurring even after vaccination in India and requires an inclusive diagnosis. Therefore, the present study was undertaken to diagnose IBD through molecular and culture methods.

One pooled sample, from each of 54 flocks having birds with IBD like symptoms, was collected. History of bird type, age and vaccination was recorded. Samples were subjected to RT-PCR, egg embryo culture and chicken fibroblast cells culture.

A total of 49669 out of 517900 (9.59 %) of birds, aging 3–6 weeks, were displaying the signs similar to IBD.In RT-PCR, 21 (38.88%) samples were found positive which belonged to11 (52.38%) vaccinated and 10 (47.62%) unvaccinated flocks.The RT- PCR positive samples were successfully cultivated for the virus through egg embryo and cell culture. The CEF culture was found least sensitive compared to egg embryo culture and RT-PCR.

Keywords

Chicken embryo fibroblast (CEF), Egg embryo inoculation, Infectious bursal disease (IBD), RT-PCR, Vaccination