Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2026
  • Volume: 60
  • Issue: 3

Detection and Molecular Analysis of Picobirnavirus and Rotavirus from Animal Faecal Samples

  • Author:
  • Mareddy Vineetha Reddy1, Vandana Gupta1*, Anju Nayak1, Nidhi Rajput2, Bhavana Gupta3, Shubhangi Warke4, Gulshan Kumar1, Sita Prasad Tiwari5
  • Total Page Count: 7
  • Page Number: 515 to 521

1Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Rewa-486 550, Madhya Pradesh, India.

2School of Wildlife Forensic and Health, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India.

3Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India.

4Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Maharashtra Animal and Fishery Sciences University, Nagpur-440 006, Maharashtra, India.

5Honourable Vice Chancellor, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India.

*Corresponding Author: Vandana Gupta, Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Rewa-486 550, Madhya Pradesh, India. Email: mareddyvineetha@gmail.com

Abstract

Picobirnavirus (PBV) is an enteric virus with potential for interspecies transmission, affecting various animal species and possibly humans. This study aimed to investigate the prevalence and molecular characterization of PBV in faecal samples from various animal species in Jabalpur, Madhya Pradesh, India. Diarrhoeic and non-diarrhoeic samples from calves (cattle and buffalo), goats, pigs, dogs and poultry were collected for analysis.

A total of 150 faecal samples, including diarrhoeic and non-diarrhoeic samples, were collected from calves (cattle and buffalo), goats, pigs, dogs and poultry. Faecal suspensions were prepared and RNA extraction was performed. Both RNA-PAGE (Polyacrylamide Gel Electrophoresis) and PCR techniques were employed to detect PBV, with PCR also used for Rotavirus detection. Various primer sets targeting the RdRp gene of segment 2 were utilized for PBV detection, while specific primers were applied for Rotavirus detection. Sequencing and phylogenetic analysis were conducted on selected samples to characterize viral genetic similarity.

Among the 150 faecal samples, 4.0% (6/150) were positive for PBV by RNA-PAGE, with three buffalo and three dog samples displaying a three-band pattern. RT-PCR using different primer sets revealed a 7.33% (11/150) prevalence of PBV, with buffalo and goats showing the highest rates. Co-infection of PBV and Rotavirus was observed in some samples, suggesting possible interactions between these viruses. Nucleotide identities of the goat isolate S14 (GenBank OP866965) with other PBV sequences in GenBank ranged from 93.40% to 95.73%.

Keywords

Interspecies transmission, Picobirnavirus, RNA-PAGE, Rotavirus, Three-band pattern