Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2026
  • Volume: 60
  • Issue: 3

ITS-1 Gene based PCR as a Diagnostic Tool for Phylogeographic Characterization of Trypanosoma evansi in Naturally Infected Equines in Agro-climatic Zones of Uttar Pradesh, India

  • Author:
  • Vijendra Kumar Pal1*, Rajender Kumar2, Amit Singh1, Hemant Kumar Singh1
  • Total Page Count: 5
  • Page Number: 534 to 538

1Department of Veterinary Parasitology, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224 229, Uttar Pradesh, India.

2ICAR-National Research Centre on Equines, Hisar-125 001, Haryana, India.

*Corresponding Author: Vijendra Kumar Pal, Department of Veterinary Parasitology, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224 229, Uttar Pradesh, India. Email: dr.vijendrakumarpal@gmail.com.

Abstract

Six representative ITS1-PCR amplified products were used for the amplifications of conserved regions of 18S and 5.8S rDNA to amplify Internal Transcribed Spacer1 (ITS1) gene of 540 bp of Typanosoma evansi isolates in equine from three agro- climatic zones of Eastern Uttar Pradesh, India.

Multiple sequence alignment was done by MAFFT online multiple sequence alignment tools by selecting the necessary parameters. The phylogenetic study of ITS1 gene revealed all the three isolates (T. evansi horse India Uttar Pradesh- North Eastern Plane Zone, T. evansi mule India Uttar Pradesh- Eastern Plane Zone and T. evansi horse India Uttar Pradesh- Vindhyan Zone) of T. evansi were clustered in single group.

Multiple alignment of nucleotide sequence of ITS1 genes showed that T. evansi isolates from Eastern region of Uttar Pradesh, Indian had > 99% nucleotide homology with isolates of camel Iran (KX898420), camel Egypt (MW603779.1) and buffalo China (FJ712715.1).

Keywords

ITS1-PCR, Multiple sequence alignment, Typanosoma evansi