1ICAR-Central Institute for Research on Buffaloes, Hisar-125 001, Haryana, India.
2ICAR-National Research Centre on Equines, Hisar-125 001, Haryana, India.
3ICAR-National Dairy Research Institute, Karnal-132 001, Haryana, India.
*Corresponding Author: Naveen Swaroop Murikipudi, ICARCentral Institute for Research on Buffaloes, Hisar-125 001, Haryana, India. Email: mnaswp@gmail.com.
Amino acids are essential for various physiological processes, including animal reproductive functions. However, amino acids function in estrus cycle has yet to be investigated. This study seeks to address this knowledge gap by exploring the role and importance of various amino acids during estrus and diestrus stages.
Blood samples were collected after confirming estrus in the animals through ultrasonography and analyzing the characteristic salivary fern patterns observed on the day of estrus. Serum then separated by centrifugation at 2000×g for 10min. Subsequently, the serum samples were processed and analyzed using LC/MS/MS with an API 3000 triple quadrupole mass spectrometry system.
Alanine, Arginine, Cystine, Glutamine, Histidine, Leucine, Isoleucine, Serine, Tryptophan, Phenylalanine and Valine levels showed significant differences (p<0.001) compared to other amino acids. Previous studies suggested L-alanine and Arginine significance in theca and granulosa cells development.
Amino acids, Buffalo, Estrus, Glycine, L-alanine, LCMS
