Department of Horticulture, CCS Haryana Agricultural University, Hisar - 125 004, India.
Efficient methods for shoot regeneration, proliferation and rooting from runner tip explants of strawberry (Fragaria X ananassa) cultivars Chandler, 0so Grande and Ofra were developed. After seven weeks of incubation maximum regeneration of 100 per cent regeneration was recorded inev. Ofra on MS medium with 4 mg I−1) BAP. On Knop's medium maximum regeneration (70.0%) was observed on K4 (Knop's + 4.0 mg l−1 IBA + 0.4 mg l−1 SAP + 0.4 mg l−1 GA3) medium. In all cultivars maximum mean number of shoots per expert was observed in Mg medium (MS + 4.0 mg l−1 BAP). Among cultivars maximum mean number of multiple shoots (13.8±3.6) and range of shoots per explant (4–36) was observed in Ofra cultivar. Maximum rooting (100%) was observed on MS media (Full or half sttength of MS basal salts) with 1.0 mg l−1 IBA. Whereas, in case of Knop's based media maximum rooting of 60.0 per cent was observed on K4 medium.
