Morphological and physiological characterization of some mutant isolates of gliocladium virens, a potential mycoparasite of sclerotial plant pathogens
Abstract
Two wild and five mutant isolates of Gliocladium virens were tested for morphological and physiological characterization. On PDA and OMA media all the isolates produced usual green coloured fluffy or submerged colonies with profuse mycelial growth except 50 Gv1 V, which showed albino colour colonies. 50 Gv1 I isolate produced comparatively large size ampuliform phialides whereas larger phialospore was produced by Gv1 isolates. All the isolates grew more rapidly on PDA than OMA medium. Irrespective of isolates, high sporulation was observed both in OMA and PDA with few exceptions. In case of biomass production the PDB medium was found superior to other media. The mutant isolates 50 Gv1 V produced higher biomass on the basis of mycelial dry weight (293.00 mg) on PDB whereas on CDB highest mycelial growth (174. 67 mg) was recorded with 50 Gv1 I isolate.
