Indian Journal of Agricultural Research

SCOPUSWeb of Science
  • Year: 2009
  • Volume: 43
  • Issue: 3

Lignocellulolytic enzyme activities and substrate degradation by Volvariella volvacea, the paddy straw mushroom/chinese mushroom

  • Author:
  • Madhu Choudhary, Shashi Dhanda, Shammi Kapoor, Giridhar Soni
  • Total Page Count: 4
  • DOI:
  • Page Number: 223 to 226

Department of Microbiology, Punjab Agricultural University, Ludhiana-141 004, India.

Abstract

All the six strains of Volvariella volvacea were investigated for their lignocellulolytic potential. The strain VvS-4 and VvS-5 found to have maximum Carboxymethyl cellulase (CMCase) and Filter paperase (FPase) activity. The cellobiase activity was comparatively much higher than CMCase and FPase activity. Thus strain VvS-4 was found to be the best strain in terms of CMCase, FPase and cellobiase activity. The strain VvS-5 gave almost similar results except cellobiase activity. The xylanase activity was found maximum in strain VvS-4 i.e. 147 IU/100ml and lesser in VvS-5 (108 IU/100ml). Xylanase activity was higher than cellulase activity when it was correlated with enzyme activities. None of the cultures showed a detectable laccase activity. The maximum cellulose and hemicellulose degradation was found in VvS-4 and VvS-5 (21.86% and 21.48%; 44.80% and 46.13% respectively) compared to all other strains of V. volvacea and the minimum degradation in fresh paddy straw and control (uninoculated). The pattern of cellulose and hemicellulose degradation was similar in VvS-4 and VvS-5.

Keywords

Lignocellulytic potential, Volvariella volvacea, Mushroom