Micropropagation of Jatropha curcas (L.)

The leaf disc were enlarged in MS medium supplemented with 5 mg/l Kn, 0.5 mg/l Kn and 5 mg/l BAP + 3 mg/l Kn, 3 mg/l BAP + 5 mg/l Kn and 5 mg/l BAP + 3 mg/l Kn. However, green callus was observed in 10 mg/l BAP + 5 mg/l IBA. Quantity of callus was further increased in sub culturing on same medium. In sub culturing when medium was supplemented with charcoal primordial shoots were seen on enlarged leaf disc. On shoot tip explants leaves were seen in all the treatments. But, in sub culturing no further growth was observed. When epicotyl implanted on MS medium supplemented with 0.5 mg/l BAP + 5 mg/l Kn and charcoal one shoot was observed. On sub culturing two roots were initiated and enlarged upto 15.4 cm. In plumule explants one leaf and one leaf with one root was observed in 0.5 mg/l BAP + 3 mg/l Kn and 0.5 mg/l BAP + 5 mg/l Kn, respectively when medium was supplemented with charcoal. In hypocotyls no shooting was there however, best rooting i.e. early, maximum number and length was observed in treatment 5 mg/l IBA + 3 mg/l NAA shocked with 500 mg/l IBA.