Department of Botany, University of Chittagong, Chittagong- 4331, Bangladesh.
*Corresponding author: Telephone: +88-031-716552, Ext.4306, Fax: +88-031-726310, E-mail: pervinakter111@yahoo.com.
In this study, attempt was made to establish a reproducible protocol via tissue culture from root and coleoptiles in three Jhum rice cultivars (cvs.) viz Hamaran, Sori, Kulbadam. Spontaneous callus induction and plant regeneration as well as variation among the cultivars were observed in different combinations on MS based media. Embryogenic calli and non embryogenic calli were found on MS based media supplemented with 2.5 mgl−1 2, 4-D; 1% Casein hydrolysate (CH) and 0.5 mgl−1 Kn respectively. Plantlet regeneration occurred on MS basal medium using 2.0 mgl−1 BAP and 0.5 mgl−1 Kn. In point of coleoptiles, cv. Hamaran displayed a good number of embryogenic calli and shoot production. Most effective response amongst the cultivars was found in coleoptiles than root while explants of root failed to reproduce plantlets through tissue culture. Finally, the plantlets were transferred to the natural condition in pot for acclimatization.
Jhum rice, Coleoptiles, Roots, Embryogenesis, Plant regeneration
