In vitro plant regeneration of Trichosanthes dioica Roxb. cv. hilli from nodal explants

An efficient protocol was established for in vitro plant regeneration from nodal explants of Trichosanthes dioica Roxb. c v. hilli. Nodal segments were cultured on semisolid MS basal medium supplemented with different concentrations and combinations of cytokinins (BAP and Kinetin) and auxin (NAA) for direct shoot regeneration. Among the various concentrations of growth regulators used, MS supplemented with 2.0mg/l BAP + 0.3mg/l NAA showed highest percentage of shoot regeneration (92.74%) and maximum number of shoots per explants (4.00 ± 0.74) from lower nodal segment used as explants after four weeks of culture. The elongated shoots showed best rooting within seven to eight days after sub culturing in half strength MS supplemented with 1.0mg/l IBA. IBA proved to be better than IAA for efficient rooting. The in vitro raised plantlets were successfully established in earthen pots filled with soil, sand and farmyard mixture, and 75% of them survived in the field conditions.