Functional assigment to asl1274 unknown protein sequence in anabaena sp. PCC7121

The genome of anabaena consists of a single chromosome and six plasmid. The chromosomal genes, location 1506484-1506880, coding for various protein shows that three protein sequences, a111268, a111273 and asl1274, are not assigned to any function i.e. unknown protein. The sequence asl1274 retrieved from CyanoBase give one oligotransport protein match with BLAST2 homology search. The SVMProt search of the same sequence gives ten functional domains of various functions including transmembrane domain. The most possible match of the sequence was found by sequential BLAST of the sequence by using MODWEB. The presence of transmembarne domain is substantiated by SOSUI analysis and point out the protein belongs to family TC 2.49 in the classification of transporters. The PDB structure with matches to PROSITE entry PS01219 reveals that the unknown protein belongs to ammonium transporter. The PROSITE gives six matches. The matches were validated by using VADAR server. The hydrophobicity profile by VADAR and 3D structure of 1U7U and 2NUU proves that the protein included in the ammonium transporter family.