Department of Microbiology, School of Life Sciences, ITM University, Gwalior-474 001, India
*Corrponding author's e-mail: kumarajayitm@gmail.com; and Address: Department of Microbiology, Kamla Raje Government PG College, Gwalior, India
Online published on 20 February, 2014.
In present study an attempt has been made to investigate the allelopathic effects at different concentrations (25, 50, 75 & 100%) of Calotropis leaves extract on seed germination, seed biomass and radicle length of Vigna sinensis. The results showed that the germination delayed at the higher concentrations, and the final germination percentage was decreased by increasing leaf extract concentration. Seed biomass was decreased with increasing the concentrations and highest concentration (100%) adversely affected the fresh as well as dry seed biomass. The seeds were highly affected at two concentrations (50 and 100%). The leaf extract also possessed the biological activity against many bacterial and fungus cultures. Phytochemical screening of the extract showed the presence of a number of bioactive constituents such as glycosides, tannins, saponins, terpenes, phenols and flavonoids etc. Hence, it could be suggested that Calotropis gigantea should not be planted close to agricultural crops due to adverse effects on their growth.
Allelopathic effect, Calotropis gigantea, Radicle length, Seed biomass, Seed germination
